EN
High-throughput screening swiftly identifies proteins interacting with a target.
Reliable results from yeast cell expression systems reflect in vivo conditions.
High sensitivity captures even weak or transient protein interactions.
Unraveling Protein Interactions
Yeast two-hybrid (Y2H) is a classic molecular biology technique for studying protein-protein interactions within cells. It was first proposed and initially established by Fields and others in 1989 during their research on the transcriptional regulation of eukaryotic genes. With the development of molecular biology, several techniques have been developed based on the yeast two-hybrid system, including yeast one-hybrid, membrane-based yeast two-hybrid, and yeast three-hybrid, which are used to study interactions between proteins, as well as between proteins and DNA/RNA/ligands. These techniques play a significant role in functional genomics and interactomics research.
Leveraging the power of omics technologies, we are proud to unveil our distinctive advantage in the scientific arena. Here's a glimpse into the essence of our excellence.
High-throughput screening swiftly identifies proteins interacting with a target.
Reliable results from yeast cell expression systems reflect in vivo conditions.
High sensitivity captures even weak or transient protein interactions.
With extensive experience in screening various types of bait genes, we design suitable screening plans.
We carefully explore the appropriate screening conditions for each bait from our customers; through one-on-one spreading and spotting verification, the probability of false positives is low; we provide the delivery of positive clone materials.
We do not skimp on the number of plates laid out, ensuring a good growth environment for positive clones.
Choose between first-generation sequencing or second-generation sequencing based on the number of positive clones.
For less common species, we can provide functional annotations for positive clones.
We can provide results images at the level of CNS articles.
| Library parameters | Gateway method for building database | SAMRT method is used to build the database |
| Library size (E. Coli) | ≥1x10^7cfu | ≥5x10^6cfu |
| Average length of insert | ≥1000bp | ≥1000bp |
| Recombination rate | ≥95% | ≥90% |
Bait: We provide the CDS sequence of the bait protein or a well-constructed plasmid vector.
Library: For the nuclear system yeast two-hybrid, a library plasmid or yeast working solution is required; for the membrane system yeast two-hybrid, a library plasmid is provided.
Singapore Global Headquarters: 112 ROBINSON ROAD #03-01
Germany: Kreuzstr.60, 40210 Düsseldorf
Singapore Global Headquarters: 112 ROBINSON ROAD #03-01
Germany: Kreuzstr.60, 40210 Düsseldorf
