EN
High-throughput screening swiftly identifies proteins interacting with a target.
Reliable results from yeast cell expression systems reflect in vivo conditions.
High sensitivity captures even weak or transient protein interactions.
Exploring Protein-DNA Interactions: The Yeast One-Hybrid Assay Technique
Yeast One-Hybrid (Y1H) is a classic molecular biology technique used to study the interactions between DNA and proteins within cells. The research direction is to use the promoter of the target gene as bait to screen for upstream proteins that regulate its expression, such as transcription factors.
The promoter DNA of the bait gene is constructed on the pAbAi vector, linearized and integrated into the genome of Y1HGold, forming the bait yeast strain. The library (prey) protein is fused with the activation domain of GAL4 on the AD vector and transferred into the yeast strain. If a certain prey protein interacts with the bait DNA, GAL4 AD will be recruited to the promoter region of the AbA resistance gene, thereby activating the expression of the AbA resistance gene. In this way, yeast cells containing the interacting protein can grow on the selective medium containing AbA antibiotics, while cells without interaction cannot grow.
Leveraging the power of omics technologies, we are proud to unveil our distinctive advantage in the scientific arena. Here's a glimpse into the essence of our excellence.
High-throughput screening swiftly identifies proteins interacting with a target.
Reliable results from yeast cell expression systems reflect in vivo conditions.
High sensitivity captures even weak or transient protein interactions.
With extensive experience in screening various types of bait genes, we design suitable screening plans.
We carefully explore the appropriate screening conditions for each bait from our customers; through one-on-one spreading and spotting verification, the probability of false positives is low; we provide the delivery of positive clone materials.
We do not skimp on the number of plates laid out, ensuring a good growth environment for positive clones.
Choose between first-generation sequencing or second-generation sequencing based on the number of positive clones.
For less common species, we can provide functional annotations for positive clones.
We can provide results images at the level of CNS articles.
| Library parameters | Gateway method for building database | SAMRT method is used to build the database |
| Library size (E. Coli) | ≥1X10^7cfu | ≥5X10^6cfu |
| Average length of insert | ≥1000bp | ≥1000bp |
| Recombination rate | ≥95% | ≥90% |
Bait: Provide the bait promoter sequence or a constructed bait plasmid.
Library: Nuclear extract library plasmid.
Singapore Global Headquarters: 112 ROBINSON ROAD #03-01
Germany: Kreuzstr.60, 40210 Düsseldorf
Singapore Global Headquarters: 112 ROBINSON ROAD #03-01
Germany: Kreuzstr.60, 40210 Düsseldorf
