Exploring Protein-DNA Interactions: The Yeast One-Hybrid Assay Technique
In the specialized field of yeast hybrid technology, Omics empower adheres to the spirit of win-win cooperation. In 2018, it joined hands with Professor Chen Fan from the Institute of Genetics, Chinese Academy of Sciences, to establish a rice transcription factor library. In the same year, it collaborated with Professor Qu Lijia from Peking University to promote the screening service of the Arabidopsis transcription factor library. In July 2022, it joined forces with Professor Ning Yuese from the Plant Protection Institute of the Chinese Academy of Agricultural Sciences to launch a rice ubiquitin library, aiming to serve more basic scientific researchers.
Leveraging the power of omics technologies, we are proud to unveil our distinctive advantage in the scientific arena. Here's a glimpse into the essence of our excellence.
Strong university-enterprise collaboration: The original cloning data comes from MSU, PlantTFDB, KOME, ABRC, RBC, ensuring the authenticity of the data.
Effective screening: The coverage rate of the ubiquitin E3s family is as high as 98.94%, and the coverage rate of the transcription factor family is over 80%.
Simplified library screening process: Full-length interacting genes of CDS can be obtained directly, without the need for repeated cloning verification.
Ubiquitin library is suitable for yeast two-hybrid; transcription factor library is suitable for yeast one-hybrid and two-hybrid screening.
With extensive experience in screening various types of bait genes, we design suitable screening plans.
We carefully explore the appropriate screening conditions for each bait from our customers; through one-on-one spreading and spotting verification, the probability of false positives is low; we provide the delivery of positive clone materials.
We do not skimp on the number of plates laid out, ensuring a good growth environment for positive clones.
Choose between first-generation sequencing or second-generation sequencing based on the number of positive clones.
For less common species, we can provide functional annotations for positive clones.
We can provide results images at the level of CNS articles.
| Family library classification | Library vector | Library strains | Bait carrier | Bait strain | Service content |
| Rice transcription factor library | PGADT7 | Y187 | Single hybrid: pAbAi Double hybrid: PGBKT7 | Single hybrid: Y1HGold Double hybrid: Y2HGold | Yeast single/double hybrid screening |
| Arabidopsis transcription factor library | PDEST22 | Y187 | Single hybrid: pAbAi Double hybrid: pDEST32/pGBKT7-Leu | Single hybrid: YIHGold Double hybrid: Y2HGold | Yeast single/double hybrid screening |
| Rice ubiquitin E3 library | PGADT7 | Y187 | Double hybrid: PGBKT7 | Double hybrid: Y2HGold | Yeast two-hybrid screening |
Singapore Global Headquarters: 112 ROBINSON ROAD #03-01
Germany: Arnold-Graffi-Haus / D85 Robert-Rössle-Straße 10 13125 Berlin
United States: 2 Goddard, Irvine, CA 92618
Hong Kong: Room 618, Building 6, Phase One, Hong Kong Science Park, No. 6 Science Park West Avenue, Pak Shek Kok, New Territories, Hong Kong
Singapore Global Headquarters: 112 ROBINSON ROAD #03-01
Germany: Arnold-Graffi-Haus / D85 Robert-Rössle-Straße 10 13125 Berlin
United States: 2 Goddard, Irvine, CA 92618
Hong Kong: Room 618, Building 6, Phase One, Hong Kong Science Park, No. 6 Science Park West Avenue, Pak Shek Kok, New Territories, Hong Kong
Singapore Global Headquarters: 112 ROBINSON ROAD #03-01
Germany: Arnold-Graffi-Haus / D85 Robert-Rössle-Straße 10 13125 Berlin
United States: 2 Goddard, Irvine, CA 92618
Hong Kong: Room 618, Building 6, Phase One, Hong Kong Science Park, No. 6 Science Park West Avenue, Pak Shek Kok, New Territories, Hong Kong
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