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Yeast Family Library Screening

Exploring Protein-DNA Interactions: The Yeast One-Hybrid Assay Technique

In the specialized field of yeast hybrid technology, Omics empower adheres to the spirit of win-win cooperation. In 2018, it joined hands with Professor Chen Fan from the Institute of Genetics, Chinese Academy of Sciences, to establish a rice transcription factor library. In the same year, it collaborated with Professor Qu Lijia from Peking University to promote the screening service of the Arabidopsis transcription factor library. In July 2022, it joined forces with Professor Ning Yuese from the Plant Protection Institute of the Chinese Academy of Agricultural Sciences to launch a rice ubiquitin library, aiming to serve more basic scientific researchers.

Omics Empower Distinctive Advantage: Cutting-Edge Solutions

Leveraging the power of omics technologies, we are proud to unveil our distinctive advantage in the scientific arena. Here's a glimpse into the essence of our excellence.

Technological Superiority

Strong university-enterprise collaboration: The original cloning data comes from MSU, PlantTFDB, KOME, ABRC, RBC, ensuring the authenticity of the data.
Effective screening: The coverage rate of the ubiquitin E3s family is as high as 98.94%, and the coverage rate of the transcription factor family is over 80%.
Simplified library screening process: Full-length interacting genes of CDS can be obtained directly, without the need for repeated cloning verification.
Ubiquitin library is suitable for yeast two-hybrid; transcription factor library is suitable for yeast one-hybrid and two-hybrid screening.

Sample Requirements of Yeast Family Library Screening

Experienced

With extensive experience in screening various types of bait genes, we design suitable screening plans.

Genuine and Reliable

We carefully explore the appropriate screening conditions for each bait from our customers; through one-on-one spreading and spotting verification, the probability of false positives is low; we provide the delivery of positive clone materials.

Large-scale Library Screening

We do not skimp on the number of plates laid out, ensuring a good growth environment for positive clones.

Multiple Sequencing Options

Choose between first-generation sequencing or second-generation sequencing based on the number of positive clones.

Professional Bioinformatics Team

For less common species, we can provide functional annotations for positive clones.

Aesthetic Experimental Images

We can provide results images at the level of CNS articles.

Parameters and Service Content

Family library classification	Library vector	Library strains	Bait carrier	Bait strain	Service content
Rice transcription factor library	PGADT7	Y187	Single hybrid: pAbAi Double hybrid: PGBKT7	Single hybrid: Y1HGold Double hybrid: Y2HGold	Yeast single/double hybrid screening

Arabidopsis transcription factor library	PDEST22	Y187	Single hybrid: pAbAi Double hybrid: pDEST32/pGBKT7-Leu	Single hybrid: YIHGold Double hybrid: Y2HGold	Yeast single/double hybrid screening

Rice ubiquitin E3 library	PGADT7	Y187	Double hybrid: PGBKT7	Double hybrid: Y2HGold	Yeast two-hybrid screening

READY TO DISCOVER MORE AT OMICS EMPOWER?

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Email:
eva.zhang@omicsempower.com
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Singapore Global Headquarters: 112 ROBINSON ROAD #03-01
Germany: Kreuzstr.60, 40210 Düsseldorf

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